safetyriskБезопасность и риск фармакотерапииSafety and Risk of Pharmacotherapy2312-78212619-1164Federal State Budgetary Institution ‘Scientific Centre for Expert Evaluation of Medicinal Products’ of the Ministry of Health of the Russian Federation (FSBI ‘SCEEMP’)10.30895/2312-7821-2023-11-3-303-321safetyrisk-373Research ArticleОТ ИССЛЕДОВАНИЙ IN VITRO К КЛИНИЧЕСКИМ ИССЛЕДОВАНИЯМFROM IN VITRO EXPERIMENTS TO CLINICAL STUDIESПрименение зебраданио (zebrafish) в доклинических исследованиях лекарственных средств: проблемы и перспективыUsing Zebrafish in Preclinical Drug Studies: Challenges and Opportunitieshttps://orcid.org/0000-0002-7525-1950КалуевА. В.KalueffA. V.

Калуев Алан Валерьевич - доктор биологических наук, профессор РАН.

Олимпийский пр-т, д. 1, пгт. Сириус, Краснодарский край, 354340; ул. Аккуратова, д. 2, Санкт-Петербург, 197341; Университетская наб., д. 7–9, Санкт-Петербург, 199034; ул. Ленинградская, д. 70, п. Песочный, Санкт-Петербург, 197758

Allan V. Kalueff - Dr. Sci. (Biol.), Professor of the Russian Academy of Sciences.

Olimpiyskiy Ave, Sirius urban-type settlement Krasnodar region 354340; Akkuratova St., St Petersburg 197341; 7–9 Universitetskaya Emb., St Petersburg 199034; 70 Leningradskaya St., Pesochny settlement, St Petersburg 197758

avkalueff@gmail.comhttps://orcid.org/0000-0002-9287-1115КотоваМ. М.KotovaM. M.

Котова Мария Михайловна.

Олимпийский пр-т, д. 1, пгт. Сириус, Краснодарский край, 354340

Maria M. Kotova

Olimpiyskiy Ave, Sirius urban-type settlement Krasnodar region 354340

kotova.maria522@yandex.ruhttps://orcid.org/0000-0002-5191-0974ИкринА. Н.IkrinA. N.

Икрин Алексей Николаевич.

Олимпийский пр-т, д. 1, пгт. Сириус, Краснодарский край, 354340

Aleksey N. Ikrin

Olimpiyskiy Ave, Sirius urban-type settlement Krasnodar region 354340

ikrin00@gmail.comhttps://orcid.org/0000-0002-5561-8583КолесниковаТ. О.KolesnikovaT. O.

Колесникова Татьяна Олеговна

Олимпийский пр-т, д. 1, пгт. Сириус, Краснодарский край, 354340

Tatiana O. Kolesnikova

Olimpiyskiy Ave, Sirius urban-type settlement Krasnodar region 354340

philimontani@yandex.ruАвтономная некоммерческая образовательная организация высшего образования «Научно-технологический университет «Сириус»; Федеральное государственное бюджетное учреждение «Национальный медицинский исследовательский центр имени В.А. Алмазова» Министерства здравоохранения Российской Федерации; Федеральное государственное бюджетное образовательное учреждение высшего образования «Санкт-Петербургский государственный университет»; Федеральное государственное бюджетное учреждение «Российский научный центр радиологии и хирургических технологий имени академика А.М. Гранова»РоссияSirius University of Science and Technology; Almazov National Medical Research Center; St Petersburg State University; A.M. Granov Russian Research Center for Radiology and Surgical TechnologiesRussian FederationАвтономная некоммерческая образовательная организация высшего образования «Научно-технологический университет «Сириус»РоссияSirius University of Science and TechnologyRussian Federation202326092023113303321Copyright © Калуев А.В., Котова М.М., Икрин А.Н., Колесникова Т.О., 20232023Калуев А.В., Котова М.М., Икрин А.Н., Колесникова Т.О.Kalueff A.V., Kotova M.M., Ikrin A.N., Kolesnikova T.O.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.risksafety.ru/jour/article/view/373Актуальность

Актуальность. В доклинических испытаниях лекарственных средств становится актуальным применение вспомогательных водных позвоночных моделей, в частности рыб зебраданио (zebrafish, Danio rerio), поскольку использование грызунов для биоскрининга лекарственных препаратов существенно ограничивают фискальная и регуляторная нагрузки.

Цель

Цель. Анализ эффективности модели зебраданио в доклинических исследованиях лекарственных средств, а также обзор текущего состояния ее использования, проблем и перспектив в этой области и определение стратегических направлений дальнейшего развития доклинического тестирования на зебраданио.

Обсуждение

Обсуждение. В работе описаны основные тесты на зебраданио, используемые для оценки общей токсичности, выживаемости эмбрионов и личинок, а также эндокринных нарушений, для широкого спектра малых молекул. Выявлены преимущества и недостатки применения зебраданио в доклинических испытаниях нейротропных соединений. Обозначены методологические подходы повышения эффективности токсикологических исследований препаратов на зебраданио. Показано, что в целом использование зебраданио постепенно закрепляется в качестве одного из этапов тестирования малых молекул в лабораториях мира.

Выводы

Выводы. Широкое внедрение данного модельного организма в исследовательскую и доклиническую практику в качестве дополнительного (вспомогательного) теста помимо исследований на грызунах позволит существенно ускорить разработку новых лекарственных препаратов, а также более полно и адекватно оценивать биологические риски воздействия химических веществ.

Scientific relevance

Scientific relevance. Since fiscal and regulatory constraints substantially limit bioscreening in rodent models, a wider implementation of additional alternative models in preclinical studies of medicines is gaining momentum. These alternative models include aquatic vertebrates, such as zebrafish (Danio rerio).

Aim

Aim. The study aimed to examine zebrafish models in terms of their performance in preclinical studies, their current uses, the challenges and opportunities in the field, and strategic directions for the development of preclinical testing in zebrafish.

Discussion

Discussion. Here, the authors summarise the key zebrafish tests that are currently used to assess a wide range of small molecules for their general and endocrine toxicity and effects on the survival of embryos and larvae. The review discusses the strengths and weaknesses of zebrafish models for preclinical testing of neurotropic agents. Additionally, the authors overview various methodological approaches to improving zebrafish toxicity testing. Overall, the use of zebrafish models is gradually becoming internationally established for laboratory testing of small molecules.

Conclusions

Conclusions. A wider implementation of zebrafish models in pharmaceutical research and preclinical testing as an additional alternative to rodents, particularly in Russia, may significantly accelerate the development of novel medicinal products and foster a more comprehensive and adequate assessment of the biological risks associated with chemical substances.

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The authors declare that there are no conflicts of interest present.